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ET 33-mi 2A---ET-mi 93B  
  ET 33-mi 20

EGFP expression in the midbrain (neurons), hindbrain (neurons), spinal cord (neurons), branchial arches and eyes.

 


 

Genomic location: Chromosome 14

The mutagenic insertion is in the exon of neurog1 (Neurogenin 1)

 

DNA sequence flanking Tol2 insertion site
 

 

 
 

5:TCTCCCAGCCCACCAATAAGGTGCGTATTGCGTCACGCAAATAACTTGAA

AGGACTTTGCACGACTGATGTTGTTTTTTGTTTCTTTTCTGGCGTATTCCTA

ACCTGATTTTGTAAATTTCTCCAGGTTATCAACAATGGAGATCGTATACTCC

GATATGGAAACCTCAAGCTGTGACTACTCCTTTTCGCACACGGATGATGAGG

ACTCGCGCAGCAGCCTCCACCCCGCGTCCCCCGCGTCCTCCTGCGGAAAACC

ACCTGCGTCTCCAGCCGGGCTCCAGCAAAAAAAAAGGCGCAGGGGGCGCGCG

AGGAACGAAACCACTGTGCACGTCGTGAAAAAAAACCGCAGGCTGAAGGCCA

ACGACCGCGAGAGGAACAGGATGCACAACCTTAACGACGCATTGGATGCTTT

GAGAAGCGTCCTGCCTGCGTTTCCTGACGACACAAAGCTGACCAAAATTGAG

ACTCTGCGCTTCGCTCACAACTACATCTGGGCACTTTCGGAGACCATCCGGA

TCGCAAACCAGAAGCAGGGCAAGTCAAGAGACGGTCCGCTGCTGCTCCCCGG

ACTAAGCTGCATGGCAGATGCACCCAGCCCCGGCAGTGACTCTTGCTCCTGG

CCGTCGGGGGCATCCTCGTCGTCTTCATCACCGTCTTACTGCAACTCAGACC

CGGGCAGCCCCGCAGCCATGGACGATTTTGGATACTTGCAAACCGACGTAGT

GTACAGCTGCCGCAACTTCGTGCCTAGCATCTATTAATGTGACTTTAAGCGT

TCTCATGTCGGTATA